教育经历：

2006/09‒2012/07：云南大学，微生物学，博士（导师：张克勤院士）

2002/09‒2006/07：鲁东大学，生物科学，学士

工作经历：

2020/11‒至今：云南师范大学，生命科学学院，教授

2015/10‒2020/10：云南师范大学，生命科学学院，副教授

2018/01‒2019/01：国家自然科学基金委员会，生命科学学部，兼聘教师

2015/09‒2016/09：美国麻省理工学院，Koch Institute，访问学者

2012/09‒2015/09：云南师范大学，生命科学学院，讲师

研究方向：

1、特殊生境微生物和酶资源的挖掘与利用；

2、污染土壤的微生物修复；

主持的科研、人才项目：

1. 国家自然科学基金面上项目：腾冲脂环酸芽孢杆菌D-1中AtCas9的结构特征及作用机制研究（32070106，2021.01‒2024.12）

2. 国家自然科学基金地区项目：腾冲脂环酸芽孢杆菌D-1中新型CRISPR/Cas9系统作用机制研究（31960023，2020.01‒2023.12）

3. 国家自然科学基金青年项目：GDSL酯酶的定向进化与分子改造研究（31600083，2017.01‒2019.12）

4. 国家自然科学基金地区项目：Asr1p在酿酒酵母细胞周期G2/M转变中的作用机制（31460022，2015.01‒2018.12）

5. 云南省应用基础研究计划重点项目：贝莱斯细菌sd降解西维因的分子机理研究（202301AS070012）；

6. 云南省应用基础研究计划面上项目：来源于腾冲脂环酸芽孢杆菌D-1中新型CRISPR/Cas系统作用机制研究（202001AT070075，2023.06‒2026.05）

7. 云南省应用基础研究计划面上项目：羧酸酯酶表面展示型全细胞催化剂构建及其对增塑剂邻苯二甲酸酯降解的功能研究（2016FB036，2016.10‒2019.12）

8. 云南省中青年学术和技术带头人（2018HB05，2018.01‒2022.12）

9. 云南省“兴滇英才支持计划”青年人才及项目（YNWR-QNBJ-2019-001，2020. 01‒2024. 12）。

代表性论文：（#Co-first authors;*Corresponding author）

1.  Xiong SC, Huang ZL, Xu W, Ding JM^*, Ni DW^*, Mu WM (2024). Recent progress in health effects and enzymatic production of epilactose, a functional lactose derivative. Trends in Food Science & Technology,144: 104338.

2.  Li YB^#, Wang X^#, Zhou NY, Ding JM* (2024). Yeast surface display technology: mechanisms, applications, and perspectives. Biotechnology Advances, 76: 108422.

3.  Xiong SC, Huang ZL, Ding JM*, Ni DW*, Mu WM (2024). Improvement of cellobiose 2-epimerase expression in Bacillus subtilis for efficient bioconversion of lactose to epilactose. International Journal of Biological Macromolecules, 280: 136063.

4.  Liu Y^#, Tang SK^#, Wang X^#, Wang XL, Tang XH, Wu Q, Huang ZX, Ding JM* (2023). A novel thermostable and salt-tolerant carboxylesterase involved in the initial aerobic degradation pathway for pyrethroids in Glycomyces salinus. Journal of Hazardous Materials, 451: 131128.

5.  Shi YJ^#, Duan M^#, Ding JM^#, Wang FQ, Bi LL, Zhang CX, Zhang YZ, Duan JY, Huang AH, Lei XL, Yin H, Zhang Y (2022). DNA topology regulates PAM-Cas9 interaction and DNA unwinding to enable near-PAMless cleavage by thermophilic Cas9. Molecular Cell, 82: 1‒16.

6.  Ding JM^#*, Zhou Y^#, Zhu H, Deng M, Gao YX, Yang YJ, Huang ZX* (2020). Characterization of EstZY: A new acetylesterase with 7-aminocephalosporanic acid deacetylase activity from Alicyclobacillus tengchongensis. International Journal of Biological Macromolecules, 148: 333‒341.

7.  Wang X^#, Nong SJ^#, Li JY, Liu Y, Wu Q, Huang ZX, Xu B*, Ding JM* (2023). Biochemical characterization of an acetylesterase from Bacillus subtilis and its application for 7-aminocephalosporanic acid deacetylation. Frontiers in Microbiology, 2023, 14: 1164815.

8.  Liu Y, Wang X, Nong SJ, Bai ZH, Han NY, Wu Q, Huang ZX, Ding JM* (2022). Display of a novel carboxylesterase CarCby on Escherichia coli cell surface for carbaryl pesticide bioremediation. Microbial Cell Factories, 21: 97.

9.  Ding JM^#*, Liu Y^#, Gao Y, Zhang C, Wang Y, Xu B, Yang Y, Wu Q, Huang Z* (2022). Biodegradation of λ-cyhalothrin through cell surface display of bacterial carboxylesterase. Chemosphere, 289: 133130.

10.  Yu Y, Yang J, Teng ZJ, Zheng LY, Sheng Q, Li PY, Fu HH, Li CY, Chen Y, Zhang YZ, Ding JM*, Chen XL* (2022). D-Alanine metabolism via d-Ala aminotransferase by a marine Gammaproteobacterium, Pseudoalteromonas sp. strain CF6-2. Applied and Environmental Microbiology, 88: e0221921.  

11.  Li J, Cheng JH, Teng ZJ, Zhang X, Chen XL, Sun ML, Wang JP, Zhang YZ, Ding JM*, Tian XM*, Zhang XY* (2022). A novel gelatinase from marine flocculibacter collagenilyticus SM1988: characterization and potential application in collagen oligopeptide-rich hydrolysate preparation. Marine Drugs, 20: 48.

12.  Gao C, Zhang N, He XY, Wang N, Zhang XY, Wang P, Chen XL, Zhang YZ, Ding JM*, Li CY* (2022). Characterization of the trimethylamine N-oxide transporter from PelagibacterStrain HTCC1062 reveals its oligotrophic niche adaption. Frontiers in Microbiology, 13: 838608.

13.  Cheng JH, Zhang XY, Wang Z, Zhang X, Liu SC, Song XY, Zhang YZ, Ding JM*, Chen XL*, Xu F* (2021). Potential of thermolysin-like protease A69 in preparation of bovine collagen peptides with moisture-retention ability and antioxidative activity. Marine Drugs, 19: 676.

14.  Ding JM^#*, Zhou Y^#, Wang CF^#, Peng Z, Mu YL, Tang XH, Huang ZX* (2020). Development of a whole-cell biocatalyst for diisobutyl phthalate degradation by functional display of a carboxylesterase on the surface of Escherichia coli. Microbial Cell Factories, 19: 114.

15.  Yin H, Song CQ, Suresh S, Kwan SY, Wu Q, Walsh S, Ding JM, Bogorad RL, Zhu LJ, Wolfe SA, Koteliansky V, Xue W, Langer R, Anderson DG (2018). Partial DNA-guided Cas9 enables genome editing with reduced off-target activity. Nature Chemical Biology, 14: 311‒316.

16.  Yin H, Song C, Suresh S, Wu Q, Walsh S, Rhym L, Mintzer E, Bolukbasi M, Zhu L, Kauffman K, Mou H, Oberholzer A, Ding JM, Kwan S, Bogorad R, Zatsepin T, Koteliansky V, Wolfe S, Xue W, Langer R, Anderson DG (2017). Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing. Nature Biotechnology, 35: 1179‒1187.

17.  Ding JM, Yu TT, Han NY, Yu JL, Li JJ, Yang YJ, Tang XH, Xu B, Zhou JP, Tang HZ*, Huang ZX* (2016). Identification and characterization of a new 7-aminocephalosporanic acid deacetylase from thermophilic bacteriumAlicyclobacillus tengchongensis. Journal of Bacteriology, 198: 311‒320.

主讲课程：（研究生、本科）：

本科生课程：微生物学

研究生课程：基因编辑技术